Hoagland's Nutrient Solution (Gamborg and Wetter, 1975)
<Solution A>
280 mg H3BO3, 340 mg MnSO4ㆍH2O, 10 mg CuSO4ㆍ5H2O, 22 mg ZnSO4ㆍ7H2O, 10 mg (NH4)6MO7O24ㆍ4H2O
Adjust volume to 100 ml with deionized H2O, Store at 4°C
<Solution B>
0.5 ml concentrated H2SO4
Adjust volume to 100 ml with deionized H2O, Store at 4°C
<Solution C>
3.36 g Na2EDTA,2.79 g FeSO4
Adjust volume to approximately 400 ml,
Heat the solution to 70°C while stirring until the colour turns yellow-brown Cool down,
adjust the volume to 500 ml
Store at 4°C
<Hoagland's Stock Solution (10x)>
4.7 g Ca(NO3)2ㆍ4H2O, 2.6 g MgSO4ㆍ7H2O, 3.3 g KNO3, 0.6 g NH4H2PO4, 5 ml solution A, 0.5 ml solution B Adjust volume to 500 ml with deionized H2O
Store at 4°C
<Hoagland's Nutrient Solution (1x)>
100 ml 10x stock solution, 5 ml solution C
Adjust volume to 1000 ml with deionized H2O
<Prepare just before use Hoagland's Nutrient Solution (0.1x)>
10 ml 10x stock solution, 0.5 ml solution C
Adjust volume to 1000 ml with deionized H2O
Prepare just before use.
